KUMADA Yoichi

FacultyMolecular Chemistry and Engineering
PositionAssociate Professor
Last Updated :2021/09/16

Profile

Degree

  • 2002/03
  • 2005/03

External Career

  • 2016/04-, University of Texas at Austin, Visiting Scholar
  • Campus Career

  • 2007/03-2007/04, Kyoto Institute of Technology, Research Associate
  • 2007/05-2014/01, Kyoto Institute of Technology, Assistant Professor
  • 2014/02-, Kyoto Institute of Technology, Associate Professor
  • Homepage

    Educational Activities

    Classes

    • Chemical Engineering Ⅰ
    • Biochemical Engineering
    • Chemical Engineering Ⅱ
    • Chemical Engineering, Advanced
    • Conversion of Materials and Energy
    • Chemistry of Biofunctional Molecules

    Syllabus

    Display

    Research Activities

    Research Field

    • Research Project:
      • Development of sensitive and rapid immunodiagnostics for virus
      • Separation of bio-pharmaceuticals by affinity chromatography using through-pore resins
    • Seeds of Knowledge:Display

    Papers

    • Efficient and robust isolation of rabbit scFv antibodies using antigen-coupled multilamellar vesicles
      Author : Yoichi Kumada, Yuya Hasegawa, Jun-ichi Horiuchi
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2021/03
      Volume,Number,Page : DOI: 10.1016/j.jbiosc.2020.10.007
    • Design and site-directed immobilization of single-chain Fv antibody to polystyrene latex beads via material-binding peptides and application to latex turbidimetric assay
      Author : Yoichi Kumada, Yohei Miyamura, Reina Tanibata, Koichi Takahashi, Shinya Ogasawara, Fumio Gondaira, Jun-ichi, Horiuchi
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2021/01
      Volume,Number,Page : 131(1), 84-89
    • Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors
      Author : Cao-An Vu, Pin-Hsien Pan, Yuh-Shyong Yang, Hardy Wai-Hong Chan, Yoichi Kumada, Wen-Yih Chen
      Journal : Sensors
      Publication Date : 2021/02
      Volume,Number,Page : 21(2), 650
    • Conformational Dynamics Contribute to Substrate Selectivity and Catalysis in Human Kynureninase
      Author : Christos S Karamitros, Kyle Murray, Yusuke Sugiyama, Yoichi Kumada, Kenneth A Johnson, George Georgiou, Sheena D'Arcy, Everett M Stone
      Journal : ACS Chemical Biology
      Publication Date : 2020/12
      Volume,Number,Page : 15, 12, 3159–3166
    • Oriented immobilization of rBC2LCN lectin for highly sensitive detection of human pluripotent stem cells using cell culture supernatants
      Author : Tateno H, Hiemori K, Minoshima F, Kiyoi K, Matoba K, Katayama J, Kumada Y
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2020/01
      Volume,Number,Page : 129・2・215-222
    • Reversal of indoleamine 2,3-dioxygenase–mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme
      Author : Todd A Triplett, Kendra C Garrison, Nicholas Marshall, Moses Donkor, John Blazeck, Candice Lamb, Ahlam Qerqez, Joseph D Dekker, Yuri Tanno, Wei-Cheng Lu, Christos S Karamitros, Kyle Ford, Bing Tan, Xiaoyan M Zhang, Karen McGovern, Silvia Coma, Yoichi Kumada, Mena S Yamany, Enrique Sentandreu, George Fromm, Stefano Tiziani, Taylor H Schreiber, Mark Manfredi, Lauren I R Ehrlich, Everett Stone & George Georgiou
      Journal : Nature Biotechnology
      Publication Date : 2018/07
      Volume,Number,Page : 36, 758–764
    • Identification and characterization of polydimethylsiloxane-binding peptides (PDMS-tag) for oriented immobilization of functional protein on a PDMS surface
      Author : Kumada Y, Otsuki R, Sakoda Y, Akai R, Matoba K, Katayama J, Kishimoto M, Horiuchi J
      Journal : Journal of Biotechnology
      Publication Date : 2016/08
      Volume,Number,Page : 236・193-198
    • Efficient preparation and site-directed immobilization of VHH antibodies by genetic fusion of poly(methylmethacrylate)-binding peptide (PMMA-tag).
      Author : Kumada Y, Kang B, Yamakawa K, Kishimoto M, Horiuchi JI
      Journal : Biotechnology Progress
      Publication Date : 2015/09
      Volume,Number,Page : 31・6・1563–1570
    • Phase analysis in single-chain variable fragment production by recombinant Pichia pastoris based on proteomics combined with multivariate statistics.
      Author : Fujiki Y, Kumada Y, Kishimoto M
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2015/01
      Volume,Number,Page : 120(2):pp.187-92
    • Site-Specific Immobilization of Recombinant Antibody Fragments through Material-Binding Peptides for the Sensitive Detection of Antigens in Enzyme Immunoassays
      Author : Y. Kumada
      Journal : Biochimica Biophysica Acta
      Publication Date : 2014/07
      Volume,Number,Page : 1844・pp.1960-1969
    • Efficient refolding and immobilization of PMMA-tag-fused single-chain Fv antibodies for sensitive immunological detection on a PMMA plate
      Author : Y. Kumada, Y. Ishikawa, Y. Fujiwara, R.Takeda, R. Miyamoto, D. Niwa, S. Momose, B. Kang, M. Kishimoto
      Journal : Journal of Immunological Methods
      Publication Date : 2014/06
      Volume,Number,Page : 411・pp.1-10
    • Identification and characterization of peptide fragments for the direct and site-specific immobilization of functional proteins onto the surface of silicon nitride
      Author : Y. Kumada, T. Ootsuka, M. Asada, S. Yoshizuka, M. Chiyama, M. Sakane, F. MD Hasan, K. Sawada, K. Okumura, M. Kishimoto
      Journal : Journal of Biotechnology
      Publication Date : 2014/04
      Volume,Number,Page : 184・pp.103-110
    • Immobilization and functional reconstitution of antibody Fab fragment by solid-phase refolding
      Author : Yoichi Kumada, K. Hamasaki, A. Nakagawa, E. Sasaki, T. Shirai, M. Okumura, M. Inoue, M. Kishimoto
      Journal : Journal of Immunological Methods
      Publication Date : 2013/12
      Volume,Number,Page : 400?401・70?77
    • Production of Antibody Fragments in Escherichia coli.
      Author : T. Katsuda, H. Sonoda, Y. Kumada, H. Yamaji
      Journal : Methods in Molecular Biology
      Publication Date : 2012/09
      Volume,Number,Page : 907・305-324
    • Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv
      Author : Y. Kumada, Y. Ohigashi, Y. Emori, K. Imamura, Y. Omura, M. Kishimoto
      Journal : Journal of Immunological Methods
      Publication Date : 2012/08
      Volume,Number,Page : 385 (1-2)・pp. 15-22
    • Screening of PC and PMMA-binding peptides for site-specific immobilization of proteins
      Author : Y. Kumada, S. Murata, Y. Ishikawa, K. Nakatsuka, M. Kishimoto
      Journal : Journal of Biotechnology
      Publication Date : 2012/07
      Volume,Number,Page : 160・222-228
    • Production of single-chain Fv?Fc fusion protein in stably transformed insect cells
      Author : H. Sonoda, Y. Kumada, T. Katsuda, H. Yamaji
      Journal : Biochemical Engineering Journal
      Publication Date : 2012/06
      Volume,Number,Page : 67・77-82
    • High-throughput, high-level production of PS-tag-fused single-chain Fvs by microplate-based culture
      Author : Y. Kumada, Y. Takase, E. Sasaki, M. Kishimoto
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2011/05
      Volume,Number,Page : 111 (5) pp. 569 ? 573.
    • Effects of cytoplasmic and periplasmic chaperones on secretory production of single-chain Fv antibody in Escherichia coli
      Author : H. Sonoda, Y. Kumada, T. Katsuda, H. Yamaji
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2011/05
      Volume,Number,Page : 111. (4) pp. 465 - 470.
    • Preparation of scFv-immobilized QCM sensor by PS-tag-mediated solid-phase refolding
      Author : Y. Kumada, E. Sasaki, M. Kishimoto
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2011/04
      Volume,Number,Page : 111・4・459-464
    • Cytoplasmic production of soluble and functional single-chain Fv-Fc fusion protein in Escherichia coli
      Author : H. Sonoda, Y. Kumada, T. Katsuda, H. Yamaji
      Journal : Biochemical Engineering Journal
      Publication Date : 2011/01
      Volume,Number,Page : 53・253-259
    • A Novel Solid-Phase Refolding Method for Preparation of ScFv-Immobilized Polystyrene Plates with High Antigen-Binding Activity
      Author : Y. Kumada, Y. Shiritani, K. Hamasaki, A. Nakagawa, E. Sasaki, M. Kishimoto
      Journal : Analytical and Bioanalytical Chemistry
      Publication Date : 2010/09
      Volume,Number,Page : 398・3・1295-1303
    • Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins
      Author : Y. Kumada, D. Kuroki, H. Yasui, T. Ohse, M. Kishimoto
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2010/06
      Volume,Number,Page : 109・6・583-587
    • Dinamic analysis of the silver staining gel image of 2-DE for protein quantification
      Author : M. Kishimoto, Y. Tatsumi, N. Tamesui, Y. Kumada, J. Horiuchi, K. Okumura
      Journal : Journal of Electrophoresis
      Publication Date : 2010/03
      Volume,Number,Page : 54・1・1-7
    • Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones.
      Author : H. Sonoda, Y. Kumada, T. Katsuda, H. Yamaji
      Journal : Protein Expression and Purification
      Publication Date : 2010/02
      Volume,Number,Page : 70・248-253
    • High biological activity of a recombinant protein immobilized onto polystyrene
      Author : Y. Kumada, Y. Shiritani, K. Hamasaki, T. Ohse, M. Kisihmoto
      Journal : Biotechnology Journal
      Publication Date : 2009/09
      Volume,Number,Page : 4・8・1190-1197
    • Direct Immobilization of Functional Single-Chain Variable Fragment Antibodies (scFvs) onto a Polystyrene Plate by Genetic Fusion of a Polystyrene-Binding Peptide (PS-tag)
      Author : Y. Kumada, K. Hamasaki, Y. Shiritani, A. Nakagawa, D. Kuroki, T. Ohse, D. H. Choi, Y. Katakura, M. Kishimoto
      Journal : Analytical and Bioanalytical Chemistry
      Publication Date : 2009/07
      Volume,Number,Page : 395・3・759-765
    • Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene binding peptide and an intelligent microtiter plate
      Author : Y. Kumada, K. Hamasaki, Y. Shiritani, T. Ohse, M. Kishimoto
      Journal : Journal of Biotechnology
      Publication Date : 2009/06
      Volume,Number,Page : 142・135-141
    • Tandem multimer expression of Angiotensin-I converting enzyme inhibitory peptide in Escherichia coli
      Author : F. Hasan, Y. Kumada, M. Terashima, T. Katsuda, S. Katoh
      Journal : Biotechnology Journal
      Publication Date : 2009/04
      Volume,Number,Page : 4・9・1345-1356
    • Refolding of single-chain Fv by use of an antigen-coupled column
      Author : K. Ikeda, Y. Kumada, T. Katsuda, H. Yamaji, S. Katoh
      Journal : Biochemical Engineering Journal
      Publication Date : 2009/03
      Volume,Number,Page : 44・289-291
    • Efficient Production of Single-Chain Fv Antibody Having Rare Codon Linkers in Fed-Batch Fermentation
      Author : Y. Kumada, Y. Sakan, H. Kajihara, M. Kihara, Y. Kikuchi, H. Yamaji, S. Katoh
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2009/01
      Volume,Number,Page : 107・1・64-68
    • Preparation of monodisperse and size-controlled poly(ethylene glycol) hydrogel nanoparticles using liposome templates
      Author : Se Yong An, Minh-Phuong Ngoc Bui, Yun Jung Nam, Kwi Nam Han, Cheng Ai Li, Jae Bum Choo, Eun Kyu Lee, Shigeo Katoh, Y. Kumada, Gi Hun Seong
      Journal : Journal of Colloid and Interface Science
      Publication Date : 2009/01
      Volume,Number,Page : 331・1・98-103
    • Fluorescence-based peptide screening using ligand peptides directly conjugated to a thiolated glass surface
      Author : C. H. Lim, H. M. Cho, J. Choo, S. Neff, A. Jungbauer, Y. Kumada, S. Katoh, E. K. Lee
      Journal : Biomedical Microdevices
      Publication Date : 2009/01
      Volume,Number,Page : 11・663-669
    • Recent Advances in Controlled Immobilization of Proteins Onto the Surface of the Solid Substrate and Its Possible Application to Proteomics
      Author : K. Nakanishi, T. Sakiyama, Y. Kumada, K. Imamura, H. Imanaka
      Journal : Current Proteomics
      Publication Date : 2008/10
      Volume,Number,Page : 5・3・161-175
    • Effect of Nucleotide Sequences of Polypeptide Linkers on Production of Soluble Single-Chain Fv Antibodies
      Author : Y. Kumada, T. Kawasaki, Y. Sakan, Y. Kikuchi, S. Katoh
      Journal : Journal of Chemical Engineering of Japan
      Publication Date : 2008/05
      Volume,Number,Page : 41・5・413-419
    • Characteristics of a liposome immunoassay on a poly(methylmethacrylate) surface
      Author : S. Y. Hwang, Y. Kumada, G. H. Seong, J. B. Choo, S. Katoh, E. K. Lee
      Journal : Analytical and Bioanalytical Chemistry
      Publication Date : 2007/12
      Volume,Number,Page : 389・2251-2257
    • Production of single-chain variable fragment (scFv) in fed-batch and continuous culture of P. pastoris by two different methanol feeding methods
      Author : S. Yamawaki, T. Matsumoto, Y. Onishi, Y. Kumada, T. Katsuda, E. K. LEE, S. Katoh
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2007/12
      Volume,Number,Page : 104・5・403-407
    • Screening of ACE-inhibitory peptides from a random peptide-displayed phage library using ACE-coupled liposomes
      Author : Y. Kumada, N. Hashimoto, F. Hasan, M. Terashima, K. Nakanishi, A. Jungbauer, S. Katoh
      Journal : Journal of Biotechnology
      Publication Date : 2007/08
      Volume,Number,Page : 131・2・144-149
    • Polypeptide linkers suitable for the efficient production of dimeric scFv in Escherichia coli
      Author : Y. Kumada, T. Kawasaki, Y. Kikuchi, S. Katoh
      Journal : Biochemical Engineering Journal
      Publication Date : 2007/07
      Volume,Number,Page : 35・2・158-165
    • Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate
      Author : Y. Kumada, C. Zhao, R. Ishimura, H. Imanaka, K. Imamura, K. Nakanishi
      Journal : Journal of Biotechnology
      Publication Date : 2007/03
      Volume,Number,Page : 128・2・354-361
    • Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface
      Author : Y. Kumada, S. Katoh, H. Imanaka, K. Imamura, K. Nakanishi
      Journal : Journal of Biotechnology
      Publication Date : 2007/01
      Volume,Number,Page : 127・2・288-299
    • ACE inhibitory activity and characteristics of tri-peptides obtained from bonito protein
      Author : F. Hasan, Y. Kobayashi, Y. Kumada, T. Katsuda, M. Terashima, S. Katoh
      Journal : Journal of Chemical Engineering of Japan
      Publication Date : 2007/01
      Volume,Number,Page : 40・1・59-62
    • Epitope screening by use of a random peptide-displayed phage library and polyclonal antibody-coupled liposomes
      Author : Y. Kumada, M. Nogami, F. Hasan, M. Terashima, S. Katoh
      Journal : Journal of Chemical Engineering of Japan
      Publication Date : 2006/12
      Volume,Number,Page : 39・11・1195-1199
    • Fragmentation of angiotensin-I converting enzyme inhibitory peptides from bonito meat under intestinal digestion conditions and their characterization
      Author : F. Hasan, Y. Kumada, N. Hashimoto, T. Katsuda, M. Terashima, S. Katoh
      Journal : Food and Bioproducts Processing
      Publication Date : 2006/06
      Volume,Number,Page : 84・C2・135-138
    • Colony lift immunoassay utilizing antibody-coupled liposomes encapsulating HRP
      Author : Y. Kumada, Y. Kumada, N. Minami, M. Nogami, S. Katoh
      Journal : Biochemical Engineering Journal
      Publication Date : 2006/02
      Volume,Number,Page : 29・1-2・98-102
    • Production kinetics of angiotensin-I converting enzyme inhibitory peptides from bonito meat in artificial gastric juice
      Author : F. Hasan, M. Kitagawa, Y. Kumada, N. Hashimoto, M. Shiiba, S. Katoh, M. Terashima
      Journal : Process Biochemistry
      Publication Date : 2006/01
      Volume,Number,Page : 41・3・505-511
    • Cloning, overexpression, purification, and characterization of o-acetylserine sulfhydrylase-B from Escherichia coli
      Author : C. Zhao, Y. Kumada, H. Imanaka, K. Imamura, K. Nakanishi
      Journal : Protein Expression and Purification
      Publication Date : 2006/01
      Volume,Number,Page : 47・2・607-613
    • Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins
      Author : Y. Kumada, Y. Tokunaga, H. Imanaka, K. Imamura, T. Sakiyama, S. Katoh, K. Nakanishi
      Journal : Biotechnology Progress
      Publication Date : 2006/01
      Volume,Number,Page : 22・2・401-405
    • On the interaction site of serine acetyltransferase in the cysteine synthase complex from Escherichia coli
      Author : C. Zhao, Y. Moriga, B. Feng, Y. Kumada, H. Imanaka, K. Imamura, K. Nakanishi
      Journal : Biochemical Biophysical Research Communications
      Publication Date : 2006/01
      Volume,Number,Page : 341・4・911-916
    • Template refolding by use of an antibody-coupled affinity column
      Author : S. Katoh,, Y. Kumada,, N. Maeshima
      Journal : Chemical Engineering and Technology
      Publication Date : 2005/11
      Volume,Number,Page : 28・11・1394-1397
    • Application of protein-coupled liposomes to effective affinity screening from phage library
      Author : Y. Kumada,, M. Nogami,, N. Minami,, M. Maehara,, S. Katoh
      Journal : Journal of Chromatography A
      Publication Date : 2005/01
      Volume,Number,Page : 1080・1・22-28
    • Characteristics of microblotting assay using immunoliposomes
      Author : Y. Kumada,, M. Maehara,, S. Katoh
      Journal : Journal of Bioscience and Bioengineering
      Publication Date : 2004/02
      Volume,Number,Page : 98・2・129-131
    • Liposome immunoblotting assay utilizing an enhanced chemiluminescent reaction improved by surfactant
      Author : Y. Kumada,, M. Maehara,, N. Minami,, S. Katoh
      Journal : Journal of Chemical Engineering of Japan
      Publication Date : 2003/08
      Volume,Number,Page : 36・8・926-931
    • Liposome immunoblotting assay using a substrate-forming precipitate inside immunoliposomes
      Author : Y. Kumada, M. Maehara, K. Tomioka, S. Katoh
      Journal : Biotechnology and Bioengineering
      Publication Date : 2002/04
      Volume,Number,Page : 8・4・414-418
    • Characteristics of liposome immunosorbent assay (LISA) using liposomes encapsulating coenzyme beta-NAD+
      Author : Y. Kumada,, K. Tomioka,, S. Katoh
      Journal : Journal of Chemical Engineering of Japan
      Publication Date : 2001/07
      Volume,Number,Page : 34・7・943-947

    Awards

    NO.DateAwardWinner
    2012/06
    2008/03


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